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Salbutamol Elisa Kit-Food safety

SPAN BIOTECH LTD.
SPAN BIOTECH LTD.
City: Shenzhen
Province/State: Guangdong
Country/Region: China
Tel: 86-755-89589611
Contact Person:
Ms Anna Lee

Salbutamol Elisa Kit-Food safety

Brand Name : SPAN
Certification : Salbutamol
Place of Origin : China
MOQ : 1 kit
Price : Negotiable
Payment Terms : T/T,Western Union,Paypal
Supply Ability : Salbutamol
Packaging Details : 96T/KIT
Model Number : Salbutamol
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Salbutamol Elisa Kit-Food Safety


INTENTED USE

Salbutamol ELISA Kit is a direct enzyme-linked immunosorbent assay for the quantitative detect the presence of Salbutamol in samples of urine and feed, animal’s tissue, pork’s live.

Sensitivity: 0.05 ppb

Detection Limit: Animal’s tissue and pork live 0.05 ppb / 0.2 ppb

Urine 0.05 ppb

Feed 2.5 ppb

Recovery Rate: Urine and Tissue 90% ± 15%

Pork’s live and feed 80% ± 15%


KIT COMPONENT

  • 1 X Microtiter plate (8 microwells X 12 removable strips)
  • 6 X Standard solutions (1 mL each): 0 ng/ mL, 0.05 ng/ mL, 0.15 ng/ mL, 0.45 ng/ mL, 1.35 ng/ mL, 4.05 ng/ mL
  • 1 X Salbutamol antibody (6 mL)
  • 1 X HRP conjugated antibody (6 mL)
  • 1 X Sample dilution buffer (30 mL)
  • 1 X Washing buffer concentrate (10 X , 50 mL)
  • 1 X Substrate A (6 mL)
  • 1 X Substrate B (6 mL)
  • 1 X Stopping solution (6 mL)
  • 1 X Product Manual
  1. MATERIALS REQUIRED BUT NOT PROVIDED
  • ELISA Microtiter plate reader equipped with 450 nm filters

- 50 – 200 μL multichannel micropipette

- 50, 100 and 200 μL precision micropipette

- Microplate washer or squeeze bottle

- Homogenizer

- Centrifuge

- Centrifugal tubes

- Distilled water

- HCl, NaOH


PREPARATION OF WORKING SOLUTIONS

Washing buffer: dilute 10X concentrate with distilled water (i.e. 1 mL + 9 mL).

Extract solution: mix 4% (m/v) NaCl Solution with 0.1 M HCl at the volumetric ratio 4:1.

Standard solution, Enzyme-linked conjugate, substrate A, substrate B and stopping solution are ready-to-use.


SAMPLE PREPARATION


Animal’s tissue and pork live

Method 1 (dilution factor: 4):

  • Homogenize the sample (free of fat) for 1 min at 10,000 r/min.
  • Weigh out 1.0 g of homogenate into a 15 mL centrifugal tube. Add 3 mL of extract solution and mix well and shake for 5 mins.
  • Do a centrifugation at 5000 r/min at 20-25 °C for 5 mins.
  • Take 1 mL of supernatant and mix it with 15μL of 1 M NaOH into polystyrene centrifugal tube. Adjust the pH value to 7.0-8.0.
  • Take 50 μL liquid from water phase for assay.

Method 2 (dilution factor: 1):

  • Homogenize the sample (free of fat) for 1 min at 10,000 r/min.
  • Weigh out 2.0 g of homogenate into a 50 mL centrifugal tube. Add 6 mL of Methyl and shake for 5 mins.
  • Do a centrifugation at 5000 r/min for 5 mins.
  • Take 1.5 mL of supernatant (organic layer) into a glass tube and dry it by 50 °C of Nitrogen flow.
  • Dissolve the residue by 0.5 mL of distilled water and add 0.5 mL of Hexane. Shake to mix well.
  • Get rid of the upper layer and take 50 μL liquid for assay.
  • 2.Urine (dilution factor 1)
  • Take 50 μL of clear urine sample directly for assay(Do centrifuge if urine is not clear). Make dilution by distilled water before assay if the concentration of Salbutamol is high.
  • 3.Feed (dilution factor 50)
  • Weigh out 2 g of comminuted feed sample into a 50 mL centrifugal tube. Add 2 mL of 1 M HCl and 16 mL of distilled water. Shake to mix well.
  • Do a centrifugation at 5000 r/min for 5 mins.
  • Take 200 μL of supernatant and mix well with 800 μL of sample dilution buffer. Shake to mix well.
  • Adjust the pH value to 7.0-8.0 by 1 M NaOH.
  • Take 50 μL for assay.

Contact Rebecca Yan

Skype: span.biotech

Email: rebecca@spanbio.com

Tel: +86(755)89589611

Fax: +86(755)89580096

Web:www.spanbio.com

www.spanbiotech.com


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